X-ray diffraction data sets for the native and mercury-derivatized protein crystals were collected on beamline X06SA at SLS and beamline BL32XU at SPring-8, using a 1-μm-wide, 15-μm-high microbeam38. Data were indexed and scaled with the programs XDS39 and SCALA40, or with DENZO and SCALEPACK … See more Chimaeras between ChR1 and ChR2 from C. reinhardtii and other algal species were subcloned into the pCGFP-EU vector21 for expression in HEK293 cells. The tobacco etch virus (TEV) protease cleavage site, the … See more C1C2 was mixed with monoolein (Sigma) in a 2:3 protein to lipid ratio (w/w). Aliquots (100 nl) of the protein-LCP mixture were spotted on a 96-well … See more The cells were transfected with 0.5 μg wild-type C1C2 or C1C2 mutants, using Fugene 6, for 30 h. The cells were then washed with PBS and fixed with 4% paraformaldehyde in PBS for 20 min at room temperature … See more HEK293 cells were cultured on poly-lysine-coated, glass-bottom culture dishes (Matsunami), and were transfected with 0.5 μg of a plasmid construct containing the GFP-tagged C1C2 or the GFP-tagged C1C2 mutants. At … See more WebThe NFPA Certified Hazard Recognition Specialist Program (CHRS) was created in 2024 after discussions with numerous fire protection stakeholder groups indicated a strong …

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7: Structure-Property Relationships - Chemistry LibreTexts

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